Regulatory

Part:BBa_K3804004

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-25)


Las promoter

Las promoter originally exists in Pseudomonas aeruginosa for quorum sensing. The inducer of this promotor is AHL-LasR dimer. Thus, this expression is regulated by the density of AHL-LasR dimer. LasR has some homologues such as LuxR and QscR. We used the part in yeast to detect Pseudomonas aeruginosa. In Pseudomonas aeruginosa PAO1, the sequence exists in upstream of lasB gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None